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Characterization of the endogenous promoters in Yarrowia lipolytica for the biomanufacturing applications
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  • Chang Wang,
  • Xueyao Lu,
  • Mingxin Ling,
  • Yingfang Liu,
  • Huizhi Lu,
  • Jiang Zhu,
  • Xiaoman Sun,
  • Yang Gu
Chang Wang
Nanjing Normal University

Corresponding Author:[email protected]

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Xueyao Lu
Nanjing Normal University
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Mingxin Ling
Nanjing Normal University
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Yingfang Liu
Nanjing Normal University
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Huizhi Lu
Nanjing Normal University
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Jiang Zhu
Nanjing Normal University
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Xiaoman Sun
Nanjing Normal University
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Yang Gu
Nanjing Normal University
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Abstract

Yarrowia lipolytica, an oleaginous yeast with the GRAS status, has been developed as a platform for the chemical production. Specifically, promoter engineering is an important approach to regulating gene expression at the transcriptional level, which is of great significance in constructing microbial cell factories. Until now, some work on the promoter study has been carried out in Y. lipolytica. However, compared with other microorganisms, such as Saccharomyces cerevisiae, Bacillus subtilis, and etc., obtained achievements still need further replenishment and development for Y. lipolytica. For this, we screened 81 endogenous promoters in Y. lipolytica, mainly involved in carbon metabolism, amino acid metabolism, and lipid metabolism. As a result, we obtained 15 strong promoters, 41 medium strength promoters, and 25 weak promoters, with the strength spanning from 0.06% to 1.60-fold of PTEF promoter. In general, our study provides a unique and available endogenous promoter library for Y. lipolytica.
Jan 2023Published in Process Biochemistry volume 124 on pages 245-252. 10.1016/j.procbio.2022.11.023