In the present study, zinc nanoparticles were green-synthesized using the aqueous extract of Abelmoschus esculentus. The chemical characterization of was studied different techniques. The anti-human breast cancer activity of ZnNPs@Abelmoschus esculentus was evaluated using MTT assay. The nanoparticles were formed in a spherical shape with a size less than 75 nm for the particle size. The treated cells with ZnNPs@Abelmoschus esculentus were assessed by MTT assay for 48h about the cytotoxicity and anti-human breast cancer properties on normal (HUVEC) and breast cancer cell lines i.e. AU-565, DU4475, and MDA-MB-157. In the antioxidant test, the IC50 of ZnNPs@Abelmoschus esculentus and BHT against DPPH free radicals were 269 and 174 µg/mL, respectively. The viability of malignant breast cell lines reduced dose-dependently in the presence of ZnNPs@Abelmoschus esculentus. The IC50 of ZnNPs@Abelmoschus esculentus were 167, 172, and 307 µg/mL against AU-565, DU4475, and MDA-MB-157 cell lines, respectively.