A chromosome-level genome supplies an insight into the distinctive
diapause characteristics in Caligula japonica
Abstract
Caligula japonica is a forestry pest due to its damage to multiple
trees. Recently, it served as a potential natural medical mesh
biomaterial in the medical industry. However, studies on karyotype
evolution and functional genomics of C. japonica are limited by the
absent of genomic resource and its several months of diapause. Here, we
conducted high-throughput sequencing of its genome and firstly obtained
the chromosome-level genome. we successfully assembled a high-quality
genome of 584,506,556bp with Contig N50 of 12 Mb and 31 chromosomes.
About 342 Mb repeat sequences were identified, accounting for 58.53% of
C. japonica genome. Genome annotation by de novo gene prediction and
homologous gene search yields 24791 protein-coding genes. We also
applied manual annotation of diapause genes in C. japonica genome. The
genome abstained in this research will not only support resource for
future study on diapause mechanism of C. japonica but also help to
progress comparative genomic analyses in Lepidoptera.