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Patterned light stimulation, does it affect neuronal activity?
  • +1
  • Nicola Kuczewski,
  • Anistasha Lightning,
  • Corinne Beurrier,
  • Marie Bourzeix
Nicola Kuczewski
CRNL, UCBL, UJM, INSERM, CNRS, U1028, UMR5292, NEUROPOP, Lyon, France

Corresponding Author:[email protected]

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Anistasha Lightning
CRNL, UCBL, UJM, INSERM, CNRS, U1028, UMR5292, NEUROPOP, Lyon, France
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Corinne Beurrier
Aix Marseille Univ, CNRS UMR 7289, Institut de Neurosciences de la Timone (INT), Marseille, France
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Marie Bourzeix
Aix Marseille Univ, CNRS UMR 7289, Institut de Neurosciences de la Timone (INT), Marseille, France.
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Abstract

Neuronal sensitivity to light stimulation can be a significant confounding factor for assays that use light to study neuronal processes, such as optogenetics and fluorescent imaging. While continuous light stimulation has been shown to be responsible for a decrease in firing activity in several neuronal subtypes, discontinuous light stimulation commonly used in optogenetic experiments is supposed to have a negligible action. In the present report, we experimentally test this theoretical prediction by evaluating the effect produced by ten of the most commonly used patterns of discontinuous light stimulation under several electrophysiological parameters.
04 Nov 2022Submitted to European Journal of Neuroscience
05 Nov 2022Submission Checks Completed
05 Nov 2022Assigned to Editor
07 Nov 2022Review(s) Completed, Editorial Evaluation Pending
14 Nov 2022Reviewer(s) Assigned
14 Dec 2022Editorial Decision: Revise Major
05 Jan 20231st Revision Received
06 Jan 2023Submission Checks Completed
06 Jan 2023Assigned to Editor
06 Jan 2023Review(s) Completed, Editorial Evaluation Pending
06 Jan 2023Reviewer(s) Assigned
26 Jan 2023Editorial Decision: Accept