Lacustrine, riverine, and spring carbonates are archives of terrestrial climate change and are extensively used to study paleoenvironments. Clumped isotope thermometry has been applied to freshwater carbonates to reconstruct temperatures, however, limited work has been done to evaluate comparative relationships between clumped isotopes and temperature in different types of modern freshwater carbonates. Therefore, in this study, we assemble an extensive calibration dataset with 135 samples of modern lacustrine, fluvial, and spring carbonates from 96 sites and constrain the relationship between independent observations of water temperature and the clumped isotopic composition of carbonates (denoted by Δ47). We restandardize and synthesize published data and report 159 new measurements of 25 samples. We derive a composite freshwater calibration and also evaluate differences in the Δ47-temperature dependence for different types of materials to examine whether material-specific calibrations may be justified. When material type is considered, there is a convergence of slopes between biological carbonates (freshwater gastropods and bivalves), micrite, biologically-mediated carbonates (microbialites and tufas), travertines, and other recently published syntheses, but statistically significant differences in intercepts between some materials, possibly due to seasonal biases, kinetic isotope effects, and/or varying degrees of biological influence. Δ47-based reconstructions of water δ18O generally yield values within 2‰ of measured water δ18O when using a material-specific calibration. We explore the implications of applying these new calibrations in reconstructing temperature in three case studies.