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Three-dimensional spheroid formation of adipose-derived stem cells improves the survival of fat transplantation by enhance their therapeutic effect
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  • Yucang He,
  • Zikai Zhang,
  • Zihao Li,
  • Min Lin,
  • Siqi Ding,
  • Hanwen Wu,
  • Fangfang Yang,
  • Zhongming Cai,
  • Tian Li,
  • Jingping Wang,
  • Chen Ke,
  • Shengsheng Pan,
  • Liqun Li
Yucang He
The First Affiliated Hospital of Wenzhou Medical University
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Zikai Zhang
The First Affiliated Hospital of Wenzhou Medical University
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Zihao Li
The First Affiliated Hospital of Wenzhou Medical University
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Min Lin
The Second Affiliated Hospital and Yuying Children's Hospital of Wenzhou Medical University
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Siqi Ding
Yiwu Central Hospital
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Hanwen Wu
The First Affiliated Hospital of Wenzhou Medical University
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Fangfang Yang
The First Affiliated Hospital of Wenzhou Medical University
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Zhongming Cai
The First Affiliated Hospital of Wenzhou Medical University
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Tian Li
The First Affiliated Hospital of Wenzhou Medical University
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Jingping Wang
The First Affiliated Hospital of Wenzhou Medical University
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Chen Ke
The First Affiliated Hospital of Wenzhou Medical University
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Shengsheng Pan
The First Affiliated Hospital of Wenzhou Medical University
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Liqun Li
The First Affiliated Hospital of Wenzhou Medical University

Corresponding Author:[email protected]

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Abstract

Adipose-derived stem cells (ADSCs) have important applications in basic research, especially in fat transplantation. Some studies have found that three-dimensional (3D) spheroids formed by mesenchymal stem cells have enhanced therapeutic potential. However, the fundamental basics of this effect are still being discussed. In this study, ADSCs were harvested from subcutaneous adipose tissues and 3D spheroids were formed by the automatic aggregation of ADSCs in a non-adhesive 6-well plate. Oxygen glucose deprivation (OGD) was used to simulate the transplantation microenvironment. We found that 3D culture of ADSCs triggered cell autophagy. After inhibiting autophagy by Chloroquine, the rates of apoptosis were increased. When the 3D ADSC-spheroids were re-planked, the number of senescent ADSCs decreased, and the proliferation ability was promoted. In addition, there were more cytokines secreted by 3D ADSC-spheroids including VEGF, IGF-1 and TGF-β. After adding the conditioned medium with human umbilical vein endothelial cells (HUVECs), 3D ADSC-spheroids were more likely to promote migration, and tube formation, stimulating the formation of new blood vessels. Fat grafting experiments in nude mice also showed that 3D ADSC-spheroids enhanced survival and neovascularization of fat grafts. These results suggested that 3D spheroids culturing of ADSCs can increase the therapeutic potential in fat transplantation.
11 Jan 2023Submitted to Biotechnology Journal
17 Jan 2023Submission Checks Completed
17 Jan 2023Assigned to Editor
18 Jan 2023Reviewer(s) Assigned
21 Mar 2023Review(s) Completed, Editorial Evaluation Pending
29 Mar 2023Editorial Decision: Revise Major
29 May 20231st Revision Received
30 May 2023Submission Checks Completed
30 May 2023Assigned to Editor
30 May 2023Reviewer(s) Assigned
31 May 2023Review(s) Completed, Editorial Evaluation Pending
01 Jun 2023Editorial Decision: Revise Minor
05 Jun 20232nd Revision Received
08 Jun 2023Submission Checks Completed
08 Jun 2023Assigned to Editor
08 Jun 2023Review(s) Completed, Editorial Evaluation Pending
14 Jun 2023Editorial Decision: Accept