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A simple and effective vascular network labeling method for transparent tissues of mice
  • +3
  • Lin Bai,
  • Yaping Wu,
  • Wenshu Dai,
  • Jie Zhang,
  • Lily Zheng,
  • Jingqiu Cheng
Lin Bai
Sichuan University West China Hospital
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Yaping Wu
Sichuan University West China Hospital
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Wenshu Dai
Sichuan University West China Hospital
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Jie Zhang
Sichuan University West China Hospital
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Lily Zheng
Hospital of Chengdu University of Traditional Chinese Medicine

Corresponding Author:[email protected]

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Jingqiu Cheng
Sichuan University West China Hospital
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Abstract

Vascular network labeling in transparent tissues provides more complete information on blood vessels. To achieve a fast and efficient method for vascular network labeling in transparent tissues, we compared various vascular labeling methods under different tissue clearing protocols. FITC-dextran labeling and CUBIC cleaning treatment were found to be the best options for vascular network labeling in cleared mouse tissues. Satisfactory labeling of vascular networks in various organs can be achieved by selecting FITC-dextran with different molecular weights and different administration methods.
09 Feb 2023Submitted to Journal of Biophotonics
09 Feb 2023Submission Checks Completed
09 Feb 2023Assigned to Editor
09 Feb 2023Review(s) Completed, Editorial Evaluation Pending
09 Feb 2023Reviewer(s) Assigned
27 Feb 2023Editorial Decision: Revise Major
28 Mar 20231st Revision Received
28 Mar 2023Reviewer(s) Assigned
28 Mar 2023Submission Checks Completed
28 Mar 2023Assigned to Editor
28 Mar 2023Review(s) Completed, Editorial Evaluation Pending
29 Mar 2023Editorial Decision: Accept