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NKG2D receptor regulates CD4 + T cell differentiation via interaction with dendritic cells in patients with juvenile idiopathic arthritis
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  • juan zhou,
  • Junyan Wang,
  • Linlin Tao,
  • Mingyue Liu,
  • Xue-Mei Tang,
  • Xiaoping Zhu
juan zhou
Guangzhou Women and Children's Medical Center
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Junyan Wang
Children's Hospital of Chongqing Medical University
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Linlin Tao
The Affiliated Hospital of Guizhou Medical University
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Mingyue Liu
Children's Hospital of Chongqing Medical University
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Xue-Mei Tang
Children's Hospital of Chongqing Medical University
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Xiaoping Zhu
The Affiliated Hospital of Guizhou Medical University

Corresponding Author:[email protected]

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Abstract

Objectives: To explore roles of NKG2D in interactions of CD4 + T cells and dendritic cells (DCs) in juvenile idiopathic arthritis (JIA) patients Methods: Peripheral blood from active JIA patients and healthy controls were used for flow cytometry assessments of NKG2D +CD4 + T cells and expressions of MICA and MICB on DCs. NKG2D genetically modified CD4 + T cells resulted from transfection with lentiviral vectors harboring NKG2D and NKG2D siRNA. ELISA measured supernatant cytokines in co-cultured CD4 + T cells and DCs. CD4 + T cell subgroups, MICA and MICB expression on DCs was determined by flow cytometry and transcription factors by real-time PCR. Results: All JIA patients had significantly higher content of CD4 +NKG2D + T cells compared to healthy controls (P < 0.01). Expression of NKG2D on CD4 + T cells, and MICA and MICB on DCs were significantly greater in articular JIA than systemic JIA (P < 0.05). NKG2D induced IL-12 and suppressed IL-10 and TGF-β from CD4 + T cells, increased IFN-γ + CD4+ T and IL-17 + CD4 + T cells, RORc and T-bet, but reduced CD25 + Foxp3 + CD4 + T cells, IL-4 + CD4 + T cells, Foxp3, and GATA3 in JIA patients (P<0.05). NKG2D increased IL-12 and T-bet by CD4 + T cells in healthy controls (P<0.05). NKG2D decreased IL-10 and increased CD83, MICA, and MICB of DCs via interaction with CD4 + T cells in JIA and control patients (P<0.05). Conclusions: NKG2D regulates differentiation of CD4 + T cells directly and the maturation of DCs indirectly. Targeting NKG2D may be a potential therapy for JIA.