Mais Alqasrawi

and 13 more

Abstract Background and Purpose Statins are essential for managing cardiovascular disease (CVD), yet adverse effects can lead to discontinuation and non-adherence. The UAE’s multiethnic population presents unique challenges for personalized medicine. Pharmacogenomic (PGx) testing could enhance statin efficacy and safety but is not commonly used in clinical practice. This EmHeart Study sub-analysis aimed to assess genetic and demographic factors associated with side effects in rosuvastatin and atorvastatin users among 675 patients, highlighting the need for PGx-guided therapy. Experimental Approach Patients were genotyped for SLCO1B1 and ABCG2 variants using real-time PCR. Data on demographics, comorbidities, and statin use were gathered from electronic records, with side effects tracked over 12 months. Chi-square tests and logistic regression analyzed associations between patient characteristics, genetic variants, and adverse effects. Key Results East Asians with the ABCG2 rs2231142 variant had a threefold increased risk of liver enzyme elevation with rosuvastatin. Atorvastatin users carrying SLCO1B1 rs4149056 had twice the risk of statin-associated muscle symptoms (SAMS), with higher rates in females and Arabs. Additionally, combining rosuvastatin with ezetimibe further increased risks of both SAMS and liver enzyme elevation. Conclusion and Implications Although PGx in statin prescribing is well-studied, it is underutilized in clinical practice. Importantly, genetic factors are not the sole determinants in physician decision-making. This study demonstrates that gender, ethnicity, and genetic testing significantly impact statin choice. Avoiding rosuvastatin in patients with liver-related risks and increasing physician awareness of these risks can optimize efficacy, safety, and adherence across diverse populations

Bassam R. Ali

and 1 more

Hereditary hemorrhagic telangiectasia (HHT) is an autosomal dominant disorder affecting 1 in 5,000-8,000 individuals. Hereditary hemorrhagic telangiectasia type 1 (HHT1) is the most common HHT and manifests as diverse vascular malformations ranging from mild symptoms such as epistaxis and mucosal and cutaneous telangiectases to severe arteriovenous malformations (AVMs) in the lungs, brain or liver. HHT1 is caused by heterozygous mutations in the ENG gene, which encodes endoglin, the TGFb homodimeric coreceptor. It was previously shown that some endoglin HHT1-causing variants failed to traffic to the plasma membrane due to their retention in the endoplasmic reticulum (ER) and consequent degradation by ER-associated degradation (ERAD). Endoglin is a homodimer formed in the ER, and we therefore hypothesized that mixed heterodimers might form between ER-retained variants and WT protein, thus hampering its maturation and trafficking to the plasma membrane causing dominant negative effects. Indeed, HA-tagged ER-retained mutants formed heterodimers with Myc-tagged WT Endoglin. Moreover, variants L32R, V105D, P165L, I271N and C363Y adversely affected the trafficking of WT endoglin by reducing its maturation and plasma membrane localization. These results strongly suggest dominant negative effects exerted by these ER-retained variants aggravating endoglin loss of function in patients expressing them in the heterozygous state with the WT allele. Moreover, this study may explain some of the variability observed among HHT1 patients due to the additional loss of function exerted by the dominant negative effects in addition to that due to haploinsufficiency. These findings might also have implications for some of the many conditions impacted by ERAD.