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Pathogen invasion enhances the abundance of predatory protists and their prey associations in the plant microbiome
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  • Min Gao,
  • Chao Xiong,
  • Kin-Ming (Clement) Tsui,
  • Lei Cai
Min Gao
Western Sydney University Hawkesbury Institute for the Environment
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Chao Xiong
Western Sydney University Hawkesbury Institute for the Environment
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Kin-Ming (Clement) Tsui
Tan Tock Seng Hospital
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Lei Cai
Institute of Microbiology Chinese Academy of Sciences

Corresponding Author:[email protected]

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Abstract

Untangling the responses of protistan communities associated with soil and plant compartments and their associations with bacterial and fungal communities to pathogen invasion are critical for understanding the ecological processes governing plant microbiome assembly. Here we examined the protistan communities across the soil–plant continuum of healthy chili peppers and those with Fusarium wilt disease (FWD) and integrated the bacterial and fungal microbiome data from our previous investigation in China. We found that FWD was associated with a significant enrichment of phagotrophic protists in roots and an increase in the proportion and connectivity of these phagotrophic protists in intra- and interkingdom networks. Specifically, FWD increased the negative correlations between phagotrophic protists (especially Cercozoa and Ciliophora) and several members of Actinobacteria, Alphaproteobacteria, and Gammaproteobacteria in the interkingdom networks. Furthermore, the microbiomes of diseased plants not only exhibit a higher relative abundance of functional genes related to bacterial anti-predator responses compared to healthy plants, but also contained a greater abundance of metagenome-assembled genomes possessing functional traits involved in this response. The increased microbial interkingdom correlations among bacteria, fungi, and protists, coupled with the enhanced effects of protists on bacteria and fungi, as well as the notable bacterial anti-predator feedback in the diseased plant microbiome, all suggest that FWD catalyzes the associations between different groups of microbiomes. These findings highlight the role of predatory protists in shaping microbial assembly and functionality through top-down forces during pathogenic stress, potentially contributing to co-evolution within these soil and plant microbiomes.
06 Jul 2023Submitted to Molecular Ecology
08 Jul 2023Submission Checks Completed
08 Jul 2023Assigned to Editor
08 Jul 2023Review(s) Completed, Editorial Evaluation Pending
10 Jul 2023Reviewer(s) Assigned
27 Sep 2023Editorial Decision: Revise Minor
25 Oct 20231st Revision Received
26 Oct 2023Submission Checks Completed
26 Oct 2023Assigned to Editor
26 Oct 2023Review(s) Completed, Editorial Evaluation Pending
27 Oct 2023Editorial Decision: Revise Minor
15 Nov 20232nd Revision Received
17 Nov 2023Submission Checks Completed
17 Nov 2023Assigned to Editor
17 Nov 2023Review(s) Completed, Editorial Evaluation Pending
20 Nov 2023Editorial Decision: Accept