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Engineering robust yeast for neutralizing-agent-free production of lactic acid from sugarcane bagasse hydrolysate
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  • Radityo Pangestu,
  • Prihardi Kahar,
  • Lutfi Kholida,
  • Urip Perwitasari,
  • Ahmad Thontowi,
  • Fahrurrozi Fahrurrozi,
  • Puspita Lisdiyanti,
  • Yopi Yopi,
  • Chiaki Ogino,
  • Bambang Prasetya,
  • Akihiko Kondo
Radityo Pangestu
Kobe University Graduate School of Engineering Faculty of Engineering
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Prihardi Kahar
Kobe University Graduate School of Engineering Faculty of Engineering
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Lutfi Kholida
National Research and Innovation Agency Republic of Indonesia
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Urip Perwitasari
National Research and Innovation Agency Republic of Indonesia
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Ahmad Thontowi
National Research and Innovation Agency Republic of Indonesia
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Fahrurrozi Fahrurrozi
National Research and Innovation Agency Republic of Indonesia
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Puspita Lisdiyanti
National Research and Innovation Agency Republic of Indonesia
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Yopi Yopi
National Research and Innovation Agency Republic of Indonesia
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Chiaki Ogino
Kobe University Graduate School of Engineering Faculty of Engineering

Corresponding Author:[email protected]

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Bambang Prasetya
National Standardization Agency of Indonesia
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Akihiko Kondo
Kobe University
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Abstract

From a sustainability standpoint, utilizing lignocellulose-based material for lactic acid production is highly advantageous. By-products generated by pre-treatment of biomass, however, tend to exhibit an inhibitory effect on microbial hosts. Supplementation of neutralizing agents also produces harmful waste for the environment. Here, we report a rapid and non-neutralized fermentation by an original strain, Saccharomyces cerevisiae BTCC3. This robust host was metabolically engineered by the double disruption of PDC1/5 and the introduction of L-LDH. Within only 15 h, 51% of glucose from sugarcane bagasse (SCB) hydrolysate was converted to lactic acid (productivity at 1.69 g·L-1·h-1) without neutralizing agent supplementation at any stage of fermentation. Cultivation using YPD medium under similar conditions achieved productivity of 3.68 g·L-1·h-1 and the strain could still generate lactic acid after several glucose-feeding treatment without neutralization. This study is the first report of lactic acid production from SCB using a genetically modified host in a process that requires neither detoxification nor neutralization. Overall, this method offers an alternative to tolerance engineering that involves extensive genetic manipulations.