Abstract
Phlebia genus is a relevant group of fungi with crucial role in numerous
ecosystems. In tropical and subtropical areas this genus allows the
efficient degradation of lignin and carbon recovery; however, the
majority of these fungal species remains undiscovered. The main purpose
of this work was to determine the enzymatic activity of extracellular
proteins of a novel Phlebia floridensis strain isolated in
Yucatan Peninsula, Mexico. The results that are reported here
demonstrate that soluble protein extract of P. floridensis can
degrade a broad spectrum of recalcitrant compounds. This induced protein
extract is able to modify not only phenolic and non-phenolic compounds,
but also anthroquinone dyes, even without addition of exogenous hydrogen
peroxide. Using LC-MS/MS, we were able to identify a novel
chloroperoxidase in enzymatic extract. As far as we know, this is the
first report about the presence of this type of enzyme in Phlebia genus.