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Unraveling the Pathogenesis of Crimean-Congo Hemorrhagic Fever: A Novel Approach via Non-Targeted Metabolomics by NMR Spectroscopy
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  • Çağdaş Dağ,
  • Oktay Göcenler,
  • Kerem Kahraman,
  • Derya Yapar,
  • Yaren Kahraman,
  • Cengizhan Büyükdağ,
  • Gülen Esken,
  • Serena Ozabrahamyan,
  • Tayfun Barlas,
  • Yüksel Karadağ,
  • Aysel Kocagül Çelikbaş,
  • Fusun Can,
  • Nurcan Baykam,
  • Mert Kuşkucu,
  • Önder Onder Ergönül
Çağdaş Dağ
Koç University

Corresponding Author:[email protected]

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Oktay Göcenler
Koç University
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Kerem Kahraman
Koç University
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Derya Yapar
Department of Infectious Diseases and Clinical Microbiology Çorum Erol Olçok Training and Research Hospital
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Yaren Kahraman
Koç University
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Cengizhan Büyükdağ
Koç University
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Gülen Esken
Koç University Isbank Center for Infectious Diseases (KUISCID) Koc University
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Serena Ozabrahamyan
The Ohio State University Wexner Medical Center
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Tayfun Barlas
Koç University Isbank Center for Infectious Diseases (KUISCID) Koc University
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Yüksel Karadağ
Department of Infectious Diseases and Clinical Microbiology Çorum Erol Olçok Training and Research Hospital
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Aysel Kocagül Çelikbaş
Department of Infectious Diseases and Clinical Microbiology Çorum Erol Olçok Training and Research Hospital
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Fusun Can
Koç University Isbank Center for Infectious Diseases (KUISCID) Koc University
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Nurcan Baykam
Department of Infectious Diseases and Clinical Microbiology Çorum Erol Olçok Training and Research Hospital
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Mert Kuşkucu
Koç University Isbank Center for Infectious Diseases (KUISCID) Koc University
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Önder Onder Ergönül
Koç University Isbank Center for Infectious Diseases (KUISCID) Koc University
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Abstract

The application of metabolomics for studying modifications in host metabolism due to viral infections has proven to be a game-changing approach. Prior to our study, only one other ‘omics’ study has been carried out that investigates the interplay between the host and CCHFV and its subsequent pathogenesis. We employed NMR spectroscopy, given its advantages in terms of reproducibility, minimal sample preparation, and capability to analyze complex biofluids. Our methodology builds upon the proven success of metabolomics in the research of other viral hemorrhagic fevers such as Ebola, Marburg, and Dengue. Our research underlines the critical role of SAH, a metabolite involved in numerous biochemical reactions. We provide new insights into the metabolic alterations occurring in CCHF patients. These alterations not only shed light on the disease’s pathogenesis but also pave the way for potential biomarkers and therapeutic targets. Among all the metabolites detected, S-Adenosyl-L-homocysteine and Carnosine stood out as the most prevalent, warranting further exploration of their roles in CCHFV pathogenesis and their potential as therapeutic targets.
17 Aug 2023Submitted to Journal of Medical Virology
17 Aug 2023Submission Checks Completed
17 Aug 2023Assigned to Editor
17 Aug 2023Review(s) Completed, Editorial Evaluation Pending
28 Aug 2023Reviewer(s) Assigned