Abstract
In recent years, environmental DNA (eDNA) has received attention from
biologists due to its sensitivity, convenience, labor and material
efficiency, and lack of damage to organisms. The extensive application
of eDNA has opened avenues for the monitoring and biodiversity
assessment of amphibians, which are frequently small and difficult to
observe in the field, in areas such as biodiversity survey assessment
and detection of specific, rare and endangered, or alien invasive
species. However, the accuracy of eDNA can be influenced by factors such
as ambient temperature, pH, and false positives or false negatives,
which makes eDNA an adjunctive tool rather than a replacement for
traditional surveys. This review provides a concise overview of the eDNA
method and its workflow, summarizes the differences between applying
eDNA for detecting amphibians and other organisms, reviews the research
progress in eDNA technology for amphibian monitoring, identifies factors
influencing detection efficiency, and discusses the challenges and
prospects of eDNA. It aims to serve as a reference for future research
on the application of eDNA in amphibian detection.