Engineering yeast lipids for production of designer biodiesel
- Benjamin Ouellet,
- Ahmad Abdel-Mawgoud
Benjamin Ouellet
Laval University Faculty of Science and Engineering
Author ProfileAbstract
Biodiesels constitute a growing class of fuel in the world which is
increasingly inclined towards more ecological and sustainable energy.
Despite their advantages, biodiesels are of limited cold flow properties
and of larger NOx emissions, which are mostly attributed to the chemical
composition of their oil feedstocks. This study presents a novel
approach to produce Genetically Engineered Biodiesel from genetically
manipulated oleaginous yeast oils for improving biodiesel properties and
performances. Using full-factorial central composite design, the best
chemical composition of an optimal biodiesel was predicted. Then, simple
and combined MFE1, PEX10 and POX2 mutants of the oleaginous yeast
Yarrowia lipolytica were constructed and showed interesting lipid
profiles whose biodiesel is predicted to have better cold flow
properties. These mutants showed also higher lipid titers by 2-3 folds
compared to the parent strain. This study provides a genetic engineering
strategy for tailor design of biodiesel properties and performance.27 Sep 2023Submitted to Biotechnology Journal 28 Sep 2023Submission Checks Completed
28 Sep 2023Assigned to Editor
29 Sep 2023Reviewer(s) Assigned
15 Oct 2023Review(s) Completed, Editorial Evaluation Pending
15 Oct 2023Editorial Decision: Revise Major