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Electroacupuncture alleviates MIRI by regulating hypothalamic paraventricular nucleus neurons projecting to the rostral ventrolateral medulla
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  • Qi Shu,
  • Jie Zhou,
  • Bin Zhang,
  • Fan Zhang,
  • Xiang Zhou,
  • Yan Wu,
  • Huiming Chang,
  • Ling Hu,
  • Ronglin Cai,
  • Qing Yu
Qi Shu
Anhui University of Chinese Medicine

Corresponding Author:[email protected]

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Jie Zhou
Anhui University of Chinese Medicine
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Bin Zhang
Anhui University of Chinese Medicine
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Fan Zhang
Anhui University of Chinese Medicine
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Xiang Zhou
Anhui University of Chinese Medicine
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Yan Wu
Anhui University of Chinese Medicine
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Huiming Chang
Anhui University of Chinese Medicine
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Ling Hu
Anhui University of Chinese Medicine
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Ronglin Cai
Anhui University of Chinese Medicine
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Qing Yu
Anhui University of Chinese Medicine
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Abstract

Accumulating evidence suggests that electroacupuncture (EA) has obevious therapeutic effects and unique advantages in alleviating myocardial ischemia-reperfusion injury (MIRI), while the underlying neuromolecular mechanisms of EA intervention for MIRI have not been fully elucidated. The aim of the study is to investigate the role of the neural pathway of hypothalamic paraventricular nucleus (PVN) neurons projecting to the rostral ventrolateral medulla (RVLM) in the alleviation of MIRI rats by EA preconditioning. MIRI models were established by ligating the left anterior descending coronary artery for 30 min followed by reperfusion for 2 h. Electrocardiogram recording, chemogenetics, enzyne-linked immunosorbent assay, multichannel physiology recording, hematoxylin-eosin and immunofluorescence staining methods were conducted to demonstrate that firing frequencies of neurons in the PVN and expression of c-Fos decreased by EA pretreatment. Meanwhile, EA preconditioning significantly reduced the levels of creatine kinase isoenzymes (CK-MB), cardiac troponin I (cTnI) and lactic dehydrogenase (LDH). Virus tracing showed a projection connection between PVN and RVLM. Inhibition of the PVN-RVLM neural pathway could replicate the protective effect of EA pretreatment on MIRI rats. However, activation of the pathway weakened the effect of EA preconditioning. EA pretreatment alleviated MIRI by regulating PVN neurons projecting to RVLM. This work provides novel evidence of EA pretreatment for improving MIRI.
20 Oct 2023Submitted to European Journal of Neuroscience
20 Oct 2023Submission Checks Completed
20 Oct 2023Assigned to Editor
20 Oct 2023Review(s) Completed, Editorial Evaluation Pending
05 Nov 2023Reviewer(s) Assigned
20 Mar 2024Submission Checks Completed
20 Mar 2024Assigned to Editor
20 Mar 2024Review(s) Completed, Editorial Evaluation Pending
22 Apr 2024Editorial Decision: Revise Major
19 May 20242nd Revision Received
13 Jul 2024Review(s) Completed, Editorial Evaluation Pending
14 Jul 2024Editorial Decision: Accept