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Caliper, contrast enhanced-ultrasound (CEUS) or laser speckle contrast imaging (LSCI): techniques to follow mice melanoma growth
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  • Nicolas Clere,
  • Adélie Mellinger,
  • Jeanne Hersant,
  • Clara Bourreau,
  • Simon Lecoq,
  • Eva Deveze,
  • Samir Henni
Nicolas Clere
Universite d'Angers Pole Sante

Corresponding Author:[email protected]

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Adélie Mellinger
Universite d'Angers Pole Sante
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Jeanne Hersant
Centre Hospitalier Universitaire d'Angers
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Clara Bourreau
Universite d'Angers Pole Sante
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Simon Lecoq
Centre Hospitalier Universitaire d'Angers
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Eva Deveze
Centre Hospitalier Universitaire d'Angers
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Samir Henni
Centre Hospitalier Universitaire d'Angers
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Abstract

Metastatic melanoma is a cancer for which vascularization is not a diagnostic criterion. The aim of this study was to evaluate the applicabilities of laser speckle contrast imaging (LSCI) and contrast enhanced ultrasound (CEUS) in a mouse-model. B16F10 cells were xenografted to C57BL/6 mice. Mice were treated with anti-PD1 or 0.9% NaCl and tumor volume was measured daily. CEUS and LSCI were performed weekly. No difference in tumor growth or median survival were observed between treated and no-treaated mice. No significant difference in tumor volume measurement comparing caliper and CEUS was observed. LSCI and CEUS analyses showed a decrease in tumor perfusion in both groups of mice. Although both CEUS and LSCI are useful for measuring tumor volume, LSCI appears to be more robust and effective for monitoring tumor microcirculation. Non-invasive investigations are needed to better predict tumor vascularization: CEUS and LSCI have a good applicability in a mice model.
23 Oct 2023Submitted to Journal of Biophotonics
23 Oct 2023Submission Checks Completed
23 Oct 2023Assigned to Editor
23 Oct 2023Review(s) Completed, Editorial Evaluation Pending
23 Oct 2023Reviewer(s) Assigned