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Genome-wide target capture baits for endangered shark species
  • +3
  • Clara Isabel Wagner,
  • Marvin Choquet,
  • Irina Smolina,
  • Cathy S Jones,
  • Leslie Noble,
  • Galice Hoarau
Clara Isabel Wagner
Nord universitet

Corresponding Author:[email protected]

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Marvin Choquet
University of Oslo Faculty of Mathematics and Natural Sciences
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Irina Smolina
Nord Universitetet
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Cathy S Jones
University of Aberdeen
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Leslie Noble
Nord universitet
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Galice Hoarau
Nord Universitetet
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Abstract

More than 30% of extant shark species are classified as threatened with extinction, yet reliable population-level data is often rare, frequently due to a lack of genomic resources. Here we present a new genome-wide marker set for endangered shark species. We developed a target gene capture bait set based on the transcriptome and genome of a Lamnid shark, and tested it on 36 shark specimens, representing seven species from three orders. Illumina read mapping and calling of Single Nucleotide Polymorphisms (SNPs) showed high target recovery rates, especially in the order Lamniformes, providing several thousand detected biallelic SNPs in each species tested. Our results show this marker set can be used for SNP-calling in a broad range of shark species, enabling detailed population assessments and other ecological and evolutionary studies.
20 Oct 2023Submitted to Molecular Ecology Resources
24 Oct 2023Submission Checks Completed
24 Oct 2023Assigned to Editor
24 Oct 2023Review(s) Completed, Editorial Evaluation Pending
05 Nov 2023Reviewer(s) Assigned