The magnetic properties in Pt/Co and Pt/Co/Mx/Pt [1] multilayers (with Mx= different caping materials), such as perpendicular magnetic anisotropy (PMA), are of particular interest for spintronic devices. In particular, it is important to obtain a strong PMA [2] on the flexible substrate in the field of wearable devices and health monitoring. However, the impact of different stacks deposition and modulation conditions on the magnetic properties of the film lacks systematic research. Here, we investigate the magnetic properties in Pt/Co/Pt, Pt/Co/RuO2/Pt structures deposited by sputtering with different hard and flexible substrates, different layer thicknesses, different deposition temperatures of the buffer layer and different capping layers. We found that the coercive field and magnetic dynamic behavior of the films vary with these factors. More-over, depositing the buffer layer under high temperature can effectively adjust the crystalline state of the films, thus affecting the performance of the films. Also, in order to break the symmetry of Pt/Co/Pt we add different thin film materials between Co and the Pt capping. In addition, we study the effect of light He+ ions irradiation on the defects and the magnetics parameters on Pt/Co/Pt. It is expected that ultra-fast magnetic moment switching can be achieved by optimizing the conditions, in order to reduce the power consumption of the device. The experimental results also show that the optimized film conditions can also obtain strong PMA on the flexible substrate. These findings help to understand magnetic properties in these structures and show the promising prospect for wearable devices and other spintronic devices.