Viola ministipulata, a newly discovered species of Violaceae from the Sanqingshan mountain in Jiangxi Province, China, is described and illustrated here. The identification is based on comprehensive morphological and molecular evidences. While the new species shares similarities with V. fukienensis W. Becker in terms of leaf color and stoloniferous habit, it can be differentiated by its smaller leaves and flowers, distinct petal coloration, stamens and lanceolate appendages of equal length, and comparatively smaller and prematurely withered stipules. Additionally, the sepals of V. ministipulata feature a distinctive basal appendage.