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Cell death and changes in primary metabolism: The onset of defence in Eucalyptus in the war against Leptocybe invasa
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  • Ismael Pinto,
  • Maíra Sarmento,
  • Auxiliadora Martins,
  • João Rocha,
  • Gloria Pinto,
  • Wagner Araújo,
  • Amadeu Soares,
  • Renato Sarmento
Ismael Pinto
Federal Institute of Tocantins (Instituto Federal de Tocantins - IFTO), Colinas do Tocantins Campus, Colinas do Tocantins, TO, Brazil; Federal University of Tocantins (Universidade Federal do Tocantins - UFT), Gurupi Campus, Gurupi, TO, Brazil
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Maíra Sarmento
Federal University of Tocantins (Universidade Federal do Tocantins - UFT), Gurupi Campus, Gurupi, TO, Brazil; University of Aveiro (Universidade de Aveiro - UA), Campus de Santiago, Department of Biology & CESAM, Aveiro, Portugal
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Auxiliadora Martins
Department of Plant Biology (Departamento de Biologia Vegetal - DBV), Federal University of Viçosa, Viçosa, MG, Brazil; Max Planck Partner Group at the Department of Plant Biology, Federal University of Viçosa, Viçosa, MG, Brazil
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João Rocha
Federal University of Tocantins (Universidade Federal do Tocantins - UFT), Gurupi Campus, Gurupi, TO, Brazil
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Gloria Pinto
University of Aveiro
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Wagner Araújo
Department of Plant Biology (Departamento de Biologia Vegetal - DBV), Federal University of Viçosa, Viçosa, MG, Brazil; Max Planck Partner Group at the Department of Plant Biology, Federal University of Viçosa, Viçosa, MG, Brazil
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Amadeu Soares
Aveiro University
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Renato Sarmento
Federal University of Tocantins

Corresponding Author:[email protected]

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Abstract

Here we investigated changes in primary metabolism and cell death around oviposition sites in two hybrid clones of Eucalyptus with different degree of resistance to Leptocybe invasa Fisher & La Salle (Hymenoptera: Eulophidae), as well as, tolerance to water deficiency. We showed that apices of the resistant clone with oviposition had a higher content of amino acids organic acids, and the compound putrescine, than those of the susceptible clone with oviposition. In contrast, apices of the resistant clone with oviposition had lower sugar content and pyruvate organic acid content than those of the susceptible clone with oviposition. Small areas of necrosis were induced around the oviposition sites in the stem apices of Eucalyptus 24 h after infestation. The resistant clone developed larger necrotic areas that showed progressive increases 24–72 h after infestation than the susceptible clone, in which cell death was significantly lower and no changes were observed in necrotic area over time. Thus, the programmed death of cells around the egg, modulated by several amino acids, is likely the first defence response of Eucalyptus against L. invasa. Our results serve as the basis for the early identification of key metabolites produced in plants in defence against galling insects.