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Genomic variation in the black-throated green warbler (Setophaga virens) suggests divergence in a disjunct Atlantic Coastal Plain population (S. v. waynei)
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  • John Carpenter,
  • Alexander Worm,
  • Than Boves,
  • Andrew Wood,
  • Joseph Poston,
  • David Toews
John Carpenter
North Carolina Wildlife Resources Commission

Corresponding Author:[email protected]

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Alexander Worm
Arkansas State University
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Than Boves
Arkansas State University
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Andrew Wood
Pennsylvania State University
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Joseph Poston
Catawba College
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David Toews
Pennsylvania State University
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Abstract

New World wood warblers (family: Parulidae) can exhibit strong phenotypic differences among species, particularly in song and plumage. However, within-species variation in these warblers—often designated as subspecies—is much more subtle and has led to significant debate over the origin, maintenance, and conservation status of populations that differ. A species that exhibits controversial subspecific status is the black-throated green warbler (Setophaga virens), a Neotropical-Nearctic migrant that breeds throughout eastern and boreal North America with several isolated populations at the margins of its range. In particular, uncertainty has lingered over the status of S. v. waynei, a disjunct population along the southeast Atlantic Coastal Plain of the United States that differs morphologically and ecologically from the nominate subspecies. Despite its unique circumstances, the subspecific status of S. v. waynei remains questionable in the absence of any population-wide genomic analyses. Here, we employ whole-genome resequencing to estimate the genetic distinctiveness among samples collected across the entirety of S. virens breeding range, including from putative S. v. waynei. Despite detecting low global differentiation (FST = 0.027) across the entire species, we observed discrete genetic clustering among S. v. waynei. Principal components analysis of genome-wide differences shows the main axis of variation separates S. v. waynei from all other S. v. virens samples. We also found that S. v. waynei is most similar to another isolated population from the Piedmont of North Carolina and detected evidence of a historical north-to-south geographic dispersal among the entire complex. Combined with previously documented ecological and morphological distinctness, our results support that S. v. waynei be considered a distinct and recognized subspecies worthy of targeted conservation efforts.