Aim: The aim of this study is to determine the relationship between spiritual well-being, anxiety and depression levels of patients receiving hemodialysis treatment. Tools and methodology: The type of this research is descriptive and correlational. The research was carried out at Gaziantep University Şahinbey Research and Training Hospital’s Hemodialysis Unit between September 26 and December 16, 2020. The sample of the study consisted of 83 patients receiving hemodialysis treatment, who met the inclusion criteria and agreed to participate in the study between the specified dates. The data were collected by using an information form, the Spiritual Well-Being Scale (FACIT-SP), Beck Anxiety and Depression Inventory. The coding and evaluation of the data were carried out with SPSS 25 statistical analysis software. Number, mean, percentile distributions, independent groups t-test, One Way Anova Variance and Pearson Correlation analyses were used to evaluate the data. Findings: It was determined that the spiritual well-being levels of the participants and the subscales of the spiritual well-being scale, meaning, peace and belief levels were moderate. It was determined that the anxiety levels of the patients receiving hemodialysis treatment were moderate, and the depression levels were mild. Conclusion: A negative significant relationship was found between the levels of meaning, peace, faith, and spiritual well-being of the patients receiving hemodialysis treatment and anxiety and depression. It was observed that as the spirituality of the individuals increased, their anxiety and depression levels decreased.