A recently castrated 4-year-old Selle Français gelding was presented for evaluation of a renal failure. Abdominal and transrectal ultrasonography revealed a large volume of anechoic free fluid within the peritoneal cavity with high creatinine concentration and a tear at the cranial pole of the bladder. Medical management was initiated for two days, then a standing laparoscopy was performed to close the defect using barbed suture. After a transient acute renal injury, the gelding recovered and was discharged from the hospital 13 days post-operatively. To our knowledge, this case is the first report using barbed sutures on the equine bladder.