Adipogenic differentiation effect of human periodontal ligament stem
cell initial cell density on autologous cells and human bone marrow
stromal cells
Abstract
Stem cells have differentiation and regulatory functions. Here, we
discuss the effect of cell culture density on stem cell proliferate,
adipogenesis and regulation ability. To investigate the effect of the
initial culture density of human periodontal ligament stem cells
(hPDLSC) on the adipogenic differentiation of autologous cells. We found
that the proliferation rate of hPDLSC increased with the initial cell
densities (0.5~8 × 10 4 cells/cm
2) increase. After adipogenic differentiation induced
by different initial cell densities of hPDLSC, we found that the mean
adipose concentration and the expression levels of lipoprotein lipase
(LPL), CCAAT/enhancer binding protein α (CEBP-α) and peroxisome
proliferator-activated receptor γ (PPAR-γ) genes all increased with
increasing cell density. To investigate the regulatory role of hPDLSCs
on the adipogenic differentiation of other cells, we used secreted
exosomes derived from hPDLSCs cultured at different initial cell
densities of 50 μg/mL to induce the adipogenic differentiation of human
bone marrow stromal cells (hBMSC). We also found that the mean adipose
concentration and the expression of LPL, CEBP-α and PPARγ genes
increased with increasing cell density, the optimal culture density was
8 × 10 4 cells/cm 2. This provides a
laboratory research basis for the application of adipogenic
differentiation of stem cells.