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Proteomic analysis of total Hydra vulgaris polyp using a combination of free-flow electrophoresis and nanoflow Liquid Chromatography-Tandem mass spectrometry (NanoLC-MS/MS)
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  • Ousmane Niakate,
  • Francois Delalande,
  • Aurelie Hirschler,
  • Frederic Blond,
  • Sarah Cianferani,
  • Alain Van Dorsselaer,
  • Christine Carapito,
  • Isabelle Audo,
  • Christina Zeitz,
  • José Alain Sahel,
  • Thierry Léveillard,
  • Ait-Ali Najate
Ousmane Niakate
Sorbonne Université, INSERM, CNRS, Institut de la Vision, 75012 Paris, France
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Francois Delalande
Laboratoire de Spectrométrie de Masse BioOrganique, IPHC, UMR 7178, CNRS-Université de Strasbourg, Strasbourg, France ; Infrastructure Nationale de Protéomique ProFI - FR2048 CNRS, Strasbourg, France.
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Aurelie Hirschler
Laboratoire de Spectrométrie de Masse BioOrganique, IPHC, UMR 7178, CNRS-Université de Strasbourg, Strasbourg, France ; Infrastructure Nationale de Protéomique ProFI - FR2048 CNRS, Strasbourg, France.
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Frederic Blond
Sorbonne Université, INSERM, CNRS, Institut de la Vision, 75012 Paris, France
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Sarah Cianferani
CNRS - IPHC
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Alain Van Dorsselaer
Laboratoire de Spectrométrie de Masse BioOrganique, IPHC, UMR 7178, CNRS-Université de Strasbourg, Strasbourg, France ; Infrastructure Nationale de Protéomique ProFI - FR2048 CNRS, Strasbourg, France.
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Christine Carapito
National Center for Scientific Research (CNRS)
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Isabelle Audo
Sorbonne Université, INSERM, CNRS, Institut de la Vision, 75012 Paris, France
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Christina Zeitz
Sorbonne Université, INSERM, CNRS, Institut de la Vision, 75012 Paris, France
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José Alain Sahel
Sorbonne Université, INSERM, CNRS, Institut de la Vision, 75012 Paris, France
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Thierry Léveillard
Sorbonne Université, INSERM, CNRS, Institut de la Vision, 75012 Paris, France
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Ait-Ali Najate
Institut de la vision

Corresponding Author:[email protected]

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Abstract

This study aimed to identify the total proteome of Hydra vulgaris, a freshwater polyp representative of the phylum Cnidaria. This organism, known for its organized nerve net, has been a favoured model due to its unique regeneration capabilities, simple bilayer architecture separated by a mesoglea, and limited cell types. While vertebrate models offer insights into molecular signaling, invertebrate like Hydra have been instrumental in uncovering the evolutionary roles of various molecules. Despite advances in genomic analyses of Hydra, a comprehensive proteomic analysis has been lacking. Here, we explored the proteome of Hydra vulgaris using a combination of gel electrophoresis and nanoflow liquid chromatography-mass spectrometry, identifying over 5,200 proteins across all cellular components. This proteomic analysis helps gain insight into the evolutionary origins and ancestral functions of proteins involved in contemporary diseases such as Alzheimer's disease or inherited retinal dystrophies. This analysis led to the identification of proteins corresponding to transporter proteins, or to endoplasmic reticulum. Such are ATPase, calcium-binding or retinal proteins with 4 linked to photoreceptors and 6 implicated in Alzheimer disease. The data set provided in this article describes the proteins identified in the body of Hydra and the processes and functions associated with a selection of them.
22 Apr 2024Submitted to PROTEOMICS
22 Apr 2024Submission Checks Completed
22 Apr 2024Assigned to Editor
05 Jun 2024Editorial Decision: Revise Minor
19 Jul 2024Review(s) Completed, Editorial Evaluation Pending
19 Jul 20241st Revision Received
22 Jul 2024Reviewer(s) Assigned
31 Jul 2024Editorial Decision: Revise Minor
01 Nov 2024Review(s) Completed, Editorial Evaluation Pending
01 Nov 20242nd Revision Received