Proteomic analysis of total Hydra vulgaris polyp using a combination of
free-flow electrophoresis and nanoflow Liquid Chromatography-Tandem mass
spectrometry (NanoLC-MS/MS)
- Ousmane Niakate,
- Francois Delalande,
- Aurelie Hirschler,
- Frederic Blond,
- Sarah Cianferani,
- Alain Van Dorsselaer,
- Christine Carapito,
- Isabelle Audo,
- Christina Zeitz,
- José Alain Sahel,
- Thierry Léveillard,
- Ait-Ali Najate
Ousmane Niakate
Sorbonne Université, INSERM, CNRS, Institut de la Vision, 75012 Paris, France
Author ProfileFrancois Delalande
Laboratoire de Spectrométrie de Masse BioOrganique, IPHC, UMR 7178, CNRS-Université de Strasbourg, Strasbourg, France ; Infrastructure Nationale de Protéomique ProFI - FR2048 CNRS, Strasbourg, France.
Author ProfileAurelie Hirschler
Laboratoire de Spectrométrie de Masse BioOrganique, IPHC, UMR 7178, CNRS-Université de Strasbourg, Strasbourg, France ; Infrastructure Nationale de Protéomique ProFI - FR2048 CNRS, Strasbourg, France.
Author ProfileFrederic Blond
Sorbonne Université, INSERM, CNRS, Institut de la Vision, 75012 Paris, France
Author ProfileAlain Van Dorsselaer
Laboratoire de Spectrométrie de Masse BioOrganique, IPHC, UMR 7178, CNRS-Université de Strasbourg, Strasbourg, France ; Infrastructure Nationale de Protéomique ProFI - FR2048 CNRS, Strasbourg, France.
Author ProfileChristine Carapito
National Center for Scientific Research (CNRS)
Author ProfileIsabelle Audo
Sorbonne Université, INSERM, CNRS, Institut de la Vision, 75012 Paris, France
Author ProfileChristina Zeitz
Sorbonne Université, INSERM, CNRS, Institut de la Vision, 75012 Paris, France
Author ProfileJosé Alain Sahel
Sorbonne Université, INSERM, CNRS, Institut de la Vision, 75012 Paris, France
Author ProfileThierry Léveillard
Sorbonne Université, INSERM, CNRS, Institut de la Vision, 75012 Paris, France
Author ProfileAbstract
This study aimed to identify the total proteome of Hydra vulgaris, a
freshwater polyp representative of the phylum Cnidaria. This organism,
known for its organized nerve net, has been a favoured model due to its
unique regeneration capabilities, simple bilayer architecture separated
by a mesoglea, and limited cell types. While vertebrate models offer
insights into molecular signaling, invertebrate like Hydra have been
instrumental in uncovering the evolutionary roles of various molecules.
Despite advances in genomic analyses of Hydra, a comprehensive proteomic
analysis has been lacking. Here, we explored the proteome of Hydra
vulgaris using a combination of gel electrophoresis and nanoflow liquid
chromatography-mass spectrometry, identifying over 5,200 proteins across
all cellular components. This proteomic analysis helps gain insight into
the evolutionary origins and ancestral functions of proteins involved in
contemporary diseases such as Alzheimer's disease or inherited retinal
dystrophies. This analysis led to the identification of proteins
corresponding to transporter proteins, or to endoplasmic reticulum. Such
are ATPase, calcium-binding or retinal proteins with 4 linked to
photoreceptors and 6 implicated in Alzheimer disease. The data set
provided in this article describes the proteins identified in the body
of Hydra and the processes and functions associated with a selection of
them.22 Apr 2024Submitted to PROTEOMICS 22 Apr 2024Submission Checks Completed
22 Apr 2024Assigned to Editor
05 Jun 2024Editorial Decision: Revise Minor
19 Jul 2024Review(s) Completed, Editorial Evaluation Pending
19 Jul 20241st Revision Received
22 Jul 2024Reviewer(s) Assigned
31 Jul 2024Editorial Decision: Revise Minor
01 Nov 2024Review(s) Completed, Editorial Evaluation Pending
01 Nov 20242nd Revision Received