Essential Site Maintenance: Authorea-powered sites will be updated circa 15:00-17:00 Eastern on Tuesday 5 November.
There should be no interruption to normal services, but please contact us at [email protected] in case you face any issues.

loading page

Impact of Postpartum Hospital Length-of-Stay on Infant Gut Microbiota: A Comprehensive Analysis of Vaginal and Caesarean birth
  • +8
  • Sabrin Bashar,
  • Hein Tun M,
  • Joseph Ting,
  • Matthew Hicks,
  • Piush Mandhane,
  • Theo J. Moraes,
  • Elinor Simons,
  • Stuart Turvey,
  • Padmaja Subbarao,
  • James Scott,
  • Anita Kozyrskyj
Sabrin Bashar
University of Alberta
Author Profile
Hein Tun M
The Chinese University of Hong Kong
Author Profile
Joseph Ting
University of Alberta
Author Profile
Matthew Hicks
University of Alberta
Author Profile
Piush Mandhane
University of Alberta
Author Profile
Theo J. Moraes
University of Toronto
Author Profile
Elinor Simons
University of Manitoba
Author Profile
Stuart Turvey
The University of British Columbia
Author Profile
Padmaja Subbarao
University of Toronto
Author Profile
James Scott
University of Toronto
Author Profile
Anita Kozyrskyj
University of Alberta

Corresponding Author:[email protected]

Author Profile

Abstract

Objectives This study aimed to assess the association between postpartum hospital length-of-stay and the composition of gut microbiota at 3 and 12 months of age in different birth modes. Design Prospective cohort of Canadian infants from the Canadian Healthy Infant Longitudinal Development (CHILD) Study born between 2008 and 2012. Setting General community. Sample 1313 infants from three study sites (Edmonton, Vancouver, and Winnipeg) of the CHILD cohort Methods Duration of hospital stay was documented in hospital records. Infants’ gut microbiota was characterized by Illumina 16S rRNA sequencing of fecal samples at 3 and 12 months. Main outcome measures Infant gut microbiota profiles. Results: In the absence of maternal intrapartum antibiotic (IAP) exposure, vaginally delivered infants (VD) with a longer hospital length-of-stay (LOS) had a higher abundance of bacteria in their gut known to cause hospital-acquired Infections (HAI), including Enterococcus at 3 months and 12 months and Citrobacter at 3 months of age. Moreover, HAI-causing bacteria Enterobacteriaceae were more abundant in later infancy in postnatal prolonged hospital stayed IAP-exposed caesarean section (CS) infants. Enterococcus or Citrobacter abundance at 3 months significantly mediated the association of LOS with low relative abundance of Bacteroidaceae and a high relative abundance of Enterococcaeae/Bacteriodaceae or Enterobacteriaceae/Bacteroidaceae ratio at 12 months of age in VD infants without IAP exposure. Conclusions LOS after birth is associated with infant gut dysbiosis. Further research is needed to explore the health outcomes of these associations.