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EFFECTS OF N-HEXANE FRACTION OF THE SEED EXTRACT OF Piper guineense ON N ω -NITRO-L-ARGININE METHYL ESTER HYDROCHLORIDE – INDUCED HYPERTENSION IN RATS
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  • Elijah Oluwatosin Olopade,
  • Adetoun Elizabeth Morakinyo,
  • Jude Oluwapelumi Alao,
  • Temitope A. Oyedepo
Elijah Oluwatosin Olopade
Adeleke University
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Adetoun Elizabeth Morakinyo
Adeleke University
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Jude Oluwapelumi Alao
Auckland University of Technology

Corresponding Author:[email protected]

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Temitope A. Oyedepo
Adeleke University
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Abstract

Aim of the Study This study aimed to investigate the effects of the n-hexane fraction of the ethanolic seed extract of PG (NFESEPG) on hypertension induced by Nω-nitro-L-arginine methyl ester (L-NAME) in rats. Specifically, the study examined the impact of NFESEPG on blood pressure, oxidative stress markers, NO concentration, angiotensin-converting enzyme (ACE) and arginase activities, and cardiac biomarkers in hypertensive rats. Materials and Methods The study involved collecting, identifying, and processing the PG plant to obtain the ethanolic seed extract. The extract was then partitioned with solvents to isolate the n-hexane fraction. Hypertension was induced in rats by oral administration of L-NAME for ten days, while concurrent treatment with NFESEPG at two doses (200 and 400 mg/kg/day) was administered orally. Blood pressure was measured using a non-invasive tail-cuff method, and various biochemical parameters were assessed. Results Treatment with both doses of NFESEPG significantly reduced systolic and diastolic blood pressure in L-NAME-induced hypertensive rats. Additionally, NFESEPG administration increased NO concentration and decreased ACE and arginase activities, malondialdehyde (MDA) levels, and cardiac biomarkers in hypertensive rats. Conclusions The findings indicate that NFESEPG effectively lowered blood pressure in hypertensive rats induced by L-NAME, potentially through mechanisms involving the modulation of oxidative stress, NO bioavailability, and cardiac biomarkers. These results suggest the therapeutic potential of NFESEPG in managing hypertension and related cardiovascular complications.
30 Apr 2024Submitted to Cell Biochemistry & Function
08 May 2024Submission Checks Completed
08 May 2024Assigned to Editor
12 May 2024Reviewer(s) Assigned
17 Jun 20241st Revision Received
17 Jun 2024Submission Checks Completed
17 Jun 2024Assigned to Editor
17 Jun 2024Review(s) Completed, Editorial Evaluation Pending
18 Jun 2024Reviewer(s) Assigned
04 Jul 2024Editorial Decision: Accept