Abstract
Abstract: In the past two decades, extensive attention has been
drawn to the use of plants for developing vaccines. Despite plenty of
efforts made on the research and development of plant-based vaccines
worldwide, there remain challenges in their productions. With the rapid
progress in genetic engineering technology, immunology and molecular
biology technology, vaccines are diversified. Meanwhile, various
production methods emerge and are improved. Currently, transgenic
technology, which involves plants as bioreactors, has become one of the
research hotspots. In general, the preparation of transgenic plant
vaccine requires the determination of target antigen, the selection of
recipient plant, the construction of plant expression vector,
transformation, expression and detection. There are various advantages
exhibited by transgenic plant vaccine platforms over insect baculovirus,
bacterial fermentation and mammalian cell protein expression platforms:
low production cost, short growth cycle, the ease of expansion, complete
protein processing system, and the prospect of inducing immune response.
The application of transgenic plant vaccines is considered promising
from two perspectives: vaccine efficacy and immune strategy. In this
paper, the basic process of transgenic plant vaccine production and the
progress in its research were reviewed to provide reference for the
development and application of transgenic plant vaccines in the future.