Root rot caused by Macrophomina phaseolina (Tassi.) is a common soil-borne disease in black gram, and its management with chemicals showed persistent toxicity and soil degradation. The existing bioagents, such as talc formulation, lead to leaching and are less productive. Hence, developing liquid bioformulation is essential to contain the disease and maximize the yield. Therefore, the current study aims to investigate the efficacy and survivability of liquid formulation of Trichoderma asperellum (Tv1 LF), metabolites against root rot of black gram, and their growth promotion. The results showed that the vigor index 4025.00 with a spore load of 1X108 cfu/ml was recorded for growth promotion and spermophere study @ 5ml /kg of seeds with Tv1 LF. The treatment T8 significantly reduced the incidence (9.1%) against control (74.3%), with an increased biomass index in the glasshouse condition. There are 32 mVOCs profiled during the tri-trophic interaction in black gram roots, and they were up or down-regulated, Viz., Mollugin, Pentadecanoic acid, Cyclopropaneoctanoic acid 2-octyl-, methyl-ester, Rhodopin, Dodecanoic acid, 1,2,3-propanetriyl ester involved in pathways like jasmonic acid, glyconeogenic and act as acyl-CoA: acyltransferase-2 inhibitor. The results of this study confirmed that Tv1 LF performed better in growth promotion and managing root rot of black gram.