Recombinant proteins represent one of the greatest achievements of modern pharmaceutical biotechnology, as they are increasingly used in almost all branches of medicine to treat a wide range of conditions. For responding to this demand, different cell engineering approaches have been developed to improve their expression. Some of them include the use of genetic elements that have the ability to prevent the silencing of the gene of interest and the generation of resistant cell lines to inhibit or avoid the Programmed Cell Death (PCD). This research focuses on the analysis of the effect of overexpression of UCOE elements and the HSP27 protein individually and together on the production of human rIFNγ in HEK293 cells. Our results show that UCOE elements of 4 kbp do not have any effect on protein production in HEK293 cells while overexpression of HSP27 prolongs the stationary phase during growth kinetics. It was also observed that the Qp of rIFNγ is 96-fold higher in clones containing the HSP27/UCOE combination compared to the clone containing only UCOE elements nor to the control cell HEK293. These results correlate with the MCP analyses, as HSP27 overexpression was observed to decrease the expression of bax, caspase 3 and cytochrome C messengers, beclin and LC3II. This study proposes the utility of a cell engineering approach based on the overexpression of the human HSP27 protein to develop the production of recombinant viruses and proteins in HEK293 cells.