Abstract
The persistence and efficacy of biocontrol agents (BCAs) in agricultural
fields are crucial for sustainable crop production. In this study, using
a novel qPCR probe protocol, we investigated the persistence of the
introduced bacterial strain Pseudomonas sivasensis CF10PS3 in the wheat
phyllosphere. The CF10PS3 strain, known for its biocontrol properties
against wheat pathogens, was applied through foliar spray, and its
persistence was monitored over seven weeks. Our qPCR assays, designed to
specifically detect CF10PS3, distinguished it from naturally occurring
P. sivasensis strains, providing precise insights into its dynamics in
the field. The experimental results indicated that CF10PS3 was already
present on the wheat leaves before application, suggesting its natural
adaptation to the foliar environment. Following initial application, a
significant increase in CF10PS3 was observed, though subsequent
environmental factors such as rain and wind caused notable fluctuations
in its population. Despite these variations, the introduced strain
showed considerable persistence, with population levels significantly
higher than those in untreated plots by the end of the study period.
This research underscores the importance of understanding BCA dynamics
in the field, highlighting the influence of environmental conditions on
their efficacy. Specific qPCR probes proved effective in monitoring
introduced strains, offering valuable insights for optimizing BCA
application strategies. Our findings contribute to developing robust
biocontrol methods, promoting sustainable agricultural practices and
enhancing crop protection.