Genomic Insights into PaYy-2: Delineating a Novel Pseudomonas aeruginosa
Phage Species
- Lihua Fang,
- Jie Ning
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Abstract
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A novel Pseudomonas aeruginosa phage, designated as PaYy-2, has been
isolated from a lake in China and its genome meticulously sequenced and
characterized. The PaYy-2 phage possesses a 92,348 base pair
double-stranded DNA genome that encodes for 168 proteins, exhibiting a
68% similarity to the genome of the known Pseudomonas phage YS35.
Phylogenetic analysis reveals that the putative RNA polymerase of PaYy-2
shares a 94% similarity with that of Pseudomonas phage SRT6, and its
putative terminase large subunit exhibits a 96% resemblance to phage
YS35, both of which are classified under the Pakpunavirus genus.
Morphological examination via transmission electron microscopy (TEM) has
provided insights into the phage’s tail and particle morphology. These
collective findings underscore the classification of PaYy-2 as a
distinct species within the Pakpunavirus genus of the family Myoviridae.Submitted to Journal of Basic Microbiology Submission Checks Completed
Assigned to Editor
Reviewer(s) Assigned
04 Jul 2024Reviewer(s) Assigned