Abstract

not-yet-known not-yet-known not-yet-known unknown Paper mulberry is a fiber resource for paper making. Washi, a traditional paper in Japan, has been produced from Broussonetia kajinoki, which is a hybrid between B. monoica and B. papyrifera. Elite strains have been vegetatively propagated and distributed within Japan. In this study, the complete chloroplast (cp) genomes of these three strains were reconstructed as pseudomolecules consisting of 160,861, 160,868, and 160,878 bp, respectively. They were compared with other strains in databases to detect insertion/deletion (INDEL) polymorphisms. There were 25 INDELs identified in these cp genomes. ISSR based MIG-seq polymorphisms were applied to determine whether varieties or regional groups can be discriminated. Although various types of cultivars exhibiting different appearances are hard to discriminate, PCA-analysis distinguished major two groups. Maternal lineages reflecting the diverse origins of Japanese paper mulberry were determined using hypervariable maternal markers. NGS-oriented nuclear markers systems revealed the heterogeneous nature of cultivars, reflecting the complex origins of Japanese B. kajinoki.