loading page

Justification of Cytoprotective and Apoptotic Action of PLGA Loaded Nedaplatin Nanoformulation against OECM-1 Cell: A Multi-Modal Analysis
  • +6
  • ilanchit chenni,
  • K. Suresh,
  • Palanivel Venkatesan,
  • Nagappan Kannappan,
  • Muthusamy Rajasekar,
  • Azamuthu Theerthu,
  • Abulkalam Asath Ahamed,
  • Ravichandran Pugazhendhi,
  • Kathiresan Sivakumar
ilanchit chenni
Annamalai University
Author Profile
K. Suresh
Annamalai University

Corresponding Author:[email protected]

Author Profile
Palanivel Venkatesan
Annamalai University
Author Profile
Nagappan Kannappan
Annamalai University
Author Profile
Muthusamy Rajasekar
Vinayaka Mission's Kirupananda Variyar Medical College & Hospitals
Author Profile
Azamuthu Theerthu
Annamalai University
Author Profile
Abulkalam Asath Ahamed
Annamalai University
Author Profile
Ravichandran Pugazhendhi
Annamalai University
Author Profile
Kathiresan Sivakumar
Madurai Kamaraj University
Author Profile

Abstract

Poly- (D, L-lactic-co-glycolic) acid-loaded nedaplatin nanoparticles (PLGA-NDP) have been used to treat oral squamous cell carcinoma (OSCC). The preparation and characterisation of PLGA-NDP, which was formed and optimised using the double emulsion technique and Box-Behnken design, were investigated in detail. Various methods, including scanning electron microscopy- energy dispersive X-ray spectroscopy, dynamic light scattering, thermogravimetric analysis-differential thermal analysis, Fourier-Transform Infrared Spectroscopy, UV-VIS spectroscopy, and in vitro drug release studies, were employed to examine the morphology, element distribution, physical state, internal structure, encapsulation efficiency, and release profile of the nanoparticles. The Box-Behnken design, which used four factors at four levels to calculate the central point, significantly improved the results. The response of the optimised condition provided an accurate prediction, and the experiment was conducted further. The design was validated, and satisfactory agreement was observed between the predicted and experimental values. The obtained results provided evidence of a new geometric construction of PLGA-loaded Nedaplatin nanoparticles with enhanced efficiency when analysed through bio-assessment, such as cytotoxicity measurement, intracellular ROS, mitochondrial dysfunction, colony formation, apoptosis-associated gene expression profile, and cell cycle analysis in OECM-1 cell lines. Therefore, these findings and techniques are useful for the development of PLGA-NDPs.