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Creating a halotolerant degrader for efficient mineralization of p-nitrophenol substituted organophosphorus pesticides in high saline wastewater
  • +4
  • Yujie Liu,
  • Weini Xiong,
  • Yuting Jiang,
  • Yan Meng,
  • Wanwan Zhao,
  • Chao Yang,
  • Ruihua Liu
Yujie Liu
Nankai University Key Laboratory of Molecular Microbiology and Technology Ministry of Education
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Weini Xiong
Nankai University Key Laboratory of Molecular Microbiology and Technology Ministry of Education
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Yuting Jiang
Nankai University Key Laboratory of Molecular Microbiology and Technology Ministry of Education
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Yan Meng
Nankai University Key Laboratory of Molecular Microbiology and Technology Ministry of Education
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Wanwan Zhao
Nankai University Key Laboratory of Molecular Microbiology and Technology Ministry of Education
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Chao Yang
Nankai University Key Laboratory of Molecular Microbiology and Technology Ministry of Education

Corresponding Author:[email protected]

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Ruihua Liu
Nankai University Department of Biochemistry and Molecular Biology
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Abstract

The bioaugmentation performance is severely reduced in the treatment of high saline pesticide wastewater because the growth and degradation activity of pesticide degraders are significantly inhibited by high salt concentrations. In this study, an artificial halotolerant degrader J9U-MP capable of mineralizing p-nitrophenol (PNP) substituted organophosphorus pesticides (OPs) [e.g., methyl parathion (MP)] was created by integrating a MP-mineralizing pathway into the genome of a salt-tolerant chassis Halomonas cupida J9. MP degradation coupled with stable isotope analysis indicated that J9U-MP was able to metabolize MP as a sole carbon source to finally produce CO 2 and H 2O in high-salt media (up to 120 g/L NaCl). J9U-MP was genetically stable during passage culture and exogenous gene integration did not negatively influence growth and metabolism of J9U-MP. A real-time monitoring system was established with enhanced green fluorescent protein (EGFP) to track the movement and activity of J9U-MP in environmental remediation. A low-oxygen tolerant system was developed by enhancing oxygen utilization, which makes J9U-MP maintain the MP-mineralizing activity under oxygen-limited conditions. More importantly, efficient mineralization of MP by J9U-MP in high saline wastewater was demonstrated. This study highlights that synthetic biology has opened up new avenues for creating stress-resistant pollutants-mineralizing microbes. Competitive advantages of J9U-MP in high-salinity and low-oxygen environments make this degrader suitable for bioaugmentation of pesticide wastewater.
16 Aug 2024Submitted to Biotechnology and Bioengineering
16 Aug 2024Submission Checks Completed
16 Aug 2024Assigned to Editor
16 Aug 2024Review(s) Completed, Editorial Evaluation Pending
18 Aug 2024Reviewer(s) Assigned
22 Oct 2024Editorial Decision: Revise Major