Essential Site Maintenance: Authorea-powered sites will be updated circa 15:00-17:00 Eastern on Tuesday 5 November.
There should be no interruption to normal services, but please contact us at [email protected] in case you face any issues.

loading page

Genetic Concordance of Staphylococcus aureus from Oropharyngeal and Sputum Cultures in People with Cystic Fibrosis
  • +15
  • Christian F. Zirbes,
  • Andries Feder,
  • Anthony J. Pamatmat,
  • Alyssa Bartels,
  • Nicholas Pitcher,
  • Alexis L. Rozen,
  • Mary Teresi,
  • Justin Krogh,
  • Margaret Regan,
  • Erin A. Arnold,
  • Jared J. Hill,
  • Lindsey D. Reinhardt,
  • Carlos Oberto,
  • Linda Boyken,
  • Valerie Reeb,
  • Ahmed M. Moustafa,
  • Paul Planet,
  • Anthony Fischer
Christian F. Zirbes
The University of Iowa Stead Family Department of Pediatrics
Author Profile
Andries Feder
The Children's Hospital of Philadelphia Research Institute
Author Profile
Anthony J. Pamatmat
The University of Iowa Stead Family Department of Pediatrics
Author Profile
Alyssa Bartels
The University of Iowa Stead Family Department of Pediatrics
Author Profile
Nicholas Pitcher
The University of Iowa Stead Family Department of Pediatrics
Author Profile
Alexis L. Rozen
The University of Iowa Stead Family Department of Pediatrics
Author Profile
Mary Teresi
The University of Iowa Stead Family Department of Pediatrics
Author Profile
Justin Krogh
The University of Iowa Stead Family Department of Pediatrics
Author Profile
Margaret Regan
The University of Iowa Stead Family Department of Pediatrics
Author Profile
Erin A. Arnold
The University of Iowa Stead Family Department of Pediatrics
Author Profile
Jared J. Hill
The University of Iowa Stead Family Department of Pediatrics
Author Profile
Lindsey D. Reinhardt
The University of Iowa Stead Family Department of Pediatrics
Author Profile
Carlos Oberto
The University of Iowa Stead Family Department of Pediatrics
Author Profile
Linda Boyken
The University of Iowa Department of Pathology
Author Profile
Valerie Reeb
The University of Iowa State Hygienic Laboratory
Author Profile
Ahmed M. Moustafa
The Children's Hospital of Philadelphia Research Institute
Author Profile
Paul Planet
The Children's Hospital of Philadelphia Research Institute
Author Profile
Anthony Fischer
The University of Iowa Stead Family Department of Pediatrics

Corresponding Author:[email protected]

Author Profile

Abstract

not-yet-known not-yet-known not-yet-known unknown Background: People with cystic fibrosis (CF) may not expectorate sputum at young ages or while on CFTR modulators. While oropharyngeal swabs are commonly used to test for lower airway pathogens, it is unknown whether Staphylococcus aureus isolated from the oropharynx represents the strain that infects the lungs. Our goal was to determine whether oropharyngeal and sputum isolates of S. aureus are genetically distinct in a cohort of patients with CF. Methods: We obtained historical S. aureus isolates from patients who intermittently expectorated sputum in 2018 and we prospectively cultured S. aureus from oropharyngeal swabs and sputum from subjects with CF between August 2020 - February 2022. We performed short-read whole genome sequencing, determined sequence type, and performed phylogenetic analysis using S. aureus core genome single nucleotide polymorphisms (SNPs). We assigned isolates from a patient to the same strain if they had the same sequence type and differed by ≤ 60 SNPs or the isolates were not disturbed by clade breaker analysis. Results: 36 subjects had S. aureus in ≥1 oropharyngeal swab and ≥1 sputum sample in 2018. In the prospective collection, 31 subjects had synchronous oropharyngeal swab and sputum collections. Although polyclonal infections were detected, sputum and oropharyngeal isolates of S. aureus typically matched the same strain within study subjects, both over the span of 2018 (31/36 patients), and when collected simultaneously from 2020-2022 (29/31 patients). Conclusions: In patients with CF who intermittently produce sputum, oropharyngeal swabs identify S. aureus with genetic and phenotypic similarity to those cultured from sputum.
28 Aug 2024Submitted to Pediatric Pulmonology
30 Aug 2024Submission Checks Completed
30 Aug 2024Assigned to Editor
30 Aug 2024Review(s) Completed, Editorial Evaluation Pending
25 Sep 2024Reviewer(s) Assigned