The Zebrafish Sperm Proteome
- Jayme Cohen-Krais,
- Carlo Martins,
- Jamie Batram,
- Zoe Crighton,
- Jean-Charles de Coriolis,
- Alice Godden,
- Daniel Marcu,
- Weronika Robak,
- Gerhard Saalbach,
- Simone Immler
Abstract
Fertilisation is based on the tight interaction between sperm and egg
and both sperm and egg proteomes are known to evolve and diverge rapidly
even between closely related species. Understanding the sperm proteome
therefore provides key insights into the mechanisms involved during
fertilisation and the fusion between sperm and egg. Despite being a
commonly used model organism for reproductive research, very little is
currently understood about the zebrafish Danio rerio sperm proteome. We
performed in depth proteomics analysis with six pooled samples
containing ejaculates from ten males each. We confidently identified
5410 proteins, from which a total of 3900 GeneIDs were generated leading
to 1720 Gene Ontology terms. This number of proteins is larger than the
number of proteins described in sperm proteomes of other species.20 Sep 2024Submitted to PROTEOMICS 25 Sep 2024Submission Checks Completed
25 Sep 2024Assigned to Editor
25 Sep 2024Review(s) Completed, Editorial Evaluation Pending
25 Sep 2024Reviewer(s) Assigned