Application of kenaf seed oil-nanostructured lipid carrier into
palm-based α-tocopherol cream for photoprotection
Abstract
The application of nanostructured lipid carrier (NLC) in UV filters
encapsulation was found to enhance its safe use. In this work, kenaf
seed oil-NLC (KSO-NLC) co-loaded with UV filters encapsulated was used
as an active ingredient in α-tocopherol cream to develop a
photoprotective prototype. It was then subjected to further analysis to
determine the physical properties storage stability and cytotoxicity.
The ratio of KSO-NLC to α-tocopherol cream was optimised based on the
SPF value using UV transmittance analyser. The physical properties of
the samples were analysed and the amount of α-tocopherol was quantified
by ultra-high performance liquid chromatography (UHPLC). The optimised
sample was then evaluated for in vitro antioxidant activities using DPPH
and ABTS assays. Meanwhile, in vitro cytotoxicity was studied on normal
human dermal fibroblast (NHDF) cell line using MTT and CCK-8 assays. The
sample produced by KSO-NLC to α-tocopherol cream in ratio 1:2 (N3)
showed SPF value > 50. Besides, the samples showed
microbial stability and sustainable α-tocopherol content upon 12 weeks
of storage. The cytotoxicity evaluation of N3 and α-tocopherol cream
(N5) showed > 100 % cell proliferation indicated there is
no side effect on the cell growth, yet it triggered the cell
proliferation with the presence of bioactive compounds. Overall, the
results herein gathered are very promising towards the development of
new green cosmetic formulations with the utilisation of KSO-NLC and
palm-based α-tocopherol cream.