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Novel cost-efficient protein-membrane based system for cells co-cultivation and modeling the intercellular communication
  • +3
  • Artem Minin,
  • Igor Blatov,
  • Valeria Lebedeva,
  • Maxim Tuchai,
  • Varvara Pozdina,
  • Ilya Zubarev
Artem Minin
M N Mikheev Institute of Metal Physics of the Ural Branch of the Russian Academy of Sciences

Corresponding Author:[email protected]

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Igor Blatov
Moscow Institute of Physics and Technology National Research University
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Valeria Lebedeva
Moscow Institute of Physics and Technology National Research University
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Maxim Tuchai
Ural Research Center for Radiation Medecine
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Varvara Pozdina
FSBIS Institute of Immunology and Physiology of the Ural Branch of the Russian Academy of Sciences
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Ilya Zubarev
Moskovskij gosudarstvennyj universitet imeni M V Lomonosova
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Abstract

In vitro systems serve as compact and manipulate models to investigate interactions between different cell types. A homogeneous population of cells predictably and uniformly responds to external factors. In a heterogeneous cell population, the effect of external growth factors is perceived in the context of intercellular interactions. Indirect cell co-cultivation allows one to observe the paracrine effects of cells and separately analyze cell populations. The article describes an application of custom-made cell co-cultivation systems based on protein membranes separated from the bottom of the vessel by the 3d printed holder or kept afloat by a magnetic field. Using the proposed co-cultivation system, we analyzed the interaction of A549 cells and fibroblasts, in the presence and absence of growth factors. During co-cultivation of cells, the expression of genes of the activation for epithelial and mesenchymal transitions decreases. The article proposes the application of a newly available system for the co-cultivation of different cell types.
13 Oct 2021Submitted to Biotechnology and Bioengineering
15 Oct 2021Submission Checks Completed
15 Oct 2021Assigned to Editor
24 Oct 2021Reviewer(s) Assigned
22 Nov 2021Editorial Decision: Revise Minor
22 Nov 2021Review(s) Completed, Editorial Evaluation Pending
19 Dec 20211st Revision Received
23 Dec 2021Submission Checks Completed
23 Dec 2021Assigned to Editor
24 Dec 2021Reviewer(s) Assigned
26 Dec 2021Editorial Decision: Accept
26 Dec 2021Review(s) Completed, Editorial Evaluation Pending