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Marine actinomycete Streptomyces variabilis S26 as biocontrol agent for vibriosis in shrimp larval rearing systems
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  • Solly Solomon,
  • Divya Babu,
  • Bhavya Kachiprath,
  • Deepthi Augustine,
  • Dhanya Kesavan,
  • Manomi Sarasan,
  • Rosamma Philip
Solly Solomon
Cochin University of Science and Technology
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Divya Babu
Cochin University of Science and Technology
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Bhavya Kachiprath
Cochin University of Science and Technology
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Deepthi Augustine
Cochin University of Science and Technology
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Dhanya Kesavan
Cochin University of Science and Technology
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Manomi Sarasan
Cochin University of Science and Technology
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Rosamma Philip
Cochin University of Science and Technology

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Abstract

Indiscriminate use of antibiotics has led to the emergence of antibiotic resistant microbes and the loss of natural flora in aquaculture systems ultimately necessitating the ban of many of the chemotherapeutants in aquaculture. Actinobacteria play a profound role in the biogeochemical cycling in the marine environment and they represent the principal source of secondary metabolites with antimicrobial property. In the present study, 98 marine derived actinomycete isolates were screened for antimicrobial activity against the common aquatic pathogens. A potent actinomycete isolate S26, identified as Streptomyces variabilis based on 16S rRNA gene sequencing was used for further study. Optimization of the fermentation medium for secondary metabolite production was carried out by response surface methodology (RSM) using DESIGN EXPERT. The ANOVA of the quadratic regression model demonstrated that the model was highly significant for the response concerned i.e., antimicrobial activity as evident from the Fisher’s F- test with a very low probability value [(P model>F) = 0.0001]. Of the 10 different solutions suggested by the software, the most suitable composition was found to be starch, 1.38 %; soy powder, 0.88 %; ammonium sulphate, 0.16 % and salinity, 27.76 ‰. S. variabilis S26 cultured in the optimized production medium was applied in the Penaeus monodon larval rearing system and the total Vibrio count and survival rate were estimated. S. variabilis S26 treatment showed a significant reduction in Vibrios and better survival in the Penaeus monodon culture system compared to the control.
23 Apr 2023Submitted to Journal of Basic Microbiology
25 Apr 2023Review(s) Completed, Editorial Evaluation Pending
25 Apr 2023Submission Checks Completed
25 Apr 2023Assigned to Editor
02 May 2023Reviewer(s) Assigned
24 May 2023Editorial Decision: Revise Minor
07 Jun 20231st Revision Received
09 Jun 2023Submission Checks Completed
09 Jun 2023Assigned to Editor
09 Jun 2023Review(s) Completed, Editorial Evaluation Pending
09 Jun 2023Reviewer(s) Assigned
25 Jul 2023Editorial Decision: Revise Major
02 Sep 20232nd Revision Received
08 Sep 2023Submission Checks Completed
08 Sep 2023Assigned to Editor
08 Sep 2023Review(s) Completed, Editorial Evaluation Pending
08 Sep 2023Reviewer(s) Assigned
03 Oct 2023Editorial Decision: Accept