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Arsenic stress mitigation using a novel plant growth promoting bacterial strain Bacillus mycoides NR5 in spinach plant (Spinacia oleracea L.)
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  • Khan M. Sarim,
  • Renu Shukla,
  • Manish S. Bhoyar,
  • Baljeet Kaur,
  • D.P. Singh
Khan M. Sarim
National Bureau of Agriculturally Important Microorganisms
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Renu Shukla
National Bureau of Agriculturally Important Microorganisms

Corresponding Author:[email protected]

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Manish S. Bhoyar
CSIR-National Botanical Research Institute
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Baljeet Kaur
Indian Agricultural Research Institute
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D.P. Singh
Indian Institute of Vegetable Research
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Abstract

The present study aimed to identify arsenic (As)-resistant bacterial strains that can be used to mitigate arsenic stress. A bacterium Bacillus mycoides NR5 having As tolerance limit of 1100 mg L -1 was isolated from Nag River, Maharashtra, India. It was also equipped with plant growth-promoting (PGP) attributes like P solubilization, siderophores, ammonia and nitrate reduction, with added antibiotic tolerance. Further, Scanning Electron Microscopy (SEM) and Transmission Electron Micrograph (TEM) suggested biosorption as possible mechanisms of arsenic tolerance. A strong peak in FTIR spectra at 3379.0 corresponding to amine in As-treated NR5 also indicated metal interaction with cell surface protein. Amplification of the arsenic reductase gene in NR5 further suggested intracellular transformation of As speciation. Moreover, the As tolerance capability of NR5 was shown in spinach plants in which the bacterium effectively mitigated 25ppm As by the production of defence-related proline molecules. Evidence from SEM, TEM and FTIR, concluded biosorption is possibly the primary mechanism of As tolerance in NR5 along with the transformation of arsenic. B. mycoides NR5 with PGP attributes, high As tolerance and antibiotic resistance mediated enhanced As tolerance in spinach plants advocated that the strain can be a better choice for As bioremediation in contaminated agricultural soil and water.
Submitted to Journal of Basic Microbiology
Submission Checks Completed
Assigned to Editor
Reviewer(s) Assigned
13 Jul 2024Reviewer(s) Assigned
07 Aug 2024Review(s) Completed, Editorial Evaluation Pending
15 Aug 2024Editorial Decision: Revise Major
29 Aug 20241st Revision Received
30 Aug 2024Submission Checks Completed
30 Aug 2024Assigned to Editor
30 Aug 2024Review(s) Completed, Editorial Evaluation Pending
11 Sep 2024Editorial Decision: Revise Minor
23 Sep 20242nd Revision Received
23 Sep 2024Submission Checks Completed
23 Sep 2024Assigned to Editor
23 Sep 2024Review(s) Completed, Editorial Evaluation Pending
28 Sep 2024Editorial Decision: Accept