In the first section of this study, the effects of two different extraction methods on the efficiency and chemical composition and antioxidant activity were compared. Extraction efficiency of supercritical fluid was significantly higher than distillation of water. The results showed that antioxidant activity increased by increasing the concentration of the essential oil. In the second part of the study, to evaluate the impact of various walls, Lepidium sativum (LS) , Lepidium perfoliatum (LP) and a combination of both (LP/LS)were used for nano-encapsulation essential oil of basil by freeze-drying. Nanoencapsulate powder was evaluated regarding efficiency, particle size, PDI, Zeta poteintial,SEM, viscosity, Brix,pH, density.humidity, aw and FTIR. In the third part, essential oil of basil in free mode at Level 1%, 5% and 20% and in encapsulated at the level of 2% and TBHQ at 0.01% added to Kilka oil without antioxidants and then the samples were place in an oven at 60 ° C for 16 days. Storage stability of kilka oil regarding peroxide, thiobarbituric and color were analyzed. The results showed that basil essential oil had antioxidant activity and reduced the oxidation process, and also nanocapsule essential oil (especially with a coating of LP gum) showed better antioxidant activity than free essential oil, so that its antioxidant activity is competitive with TBHQ synthetic antioxidants. Finally, we can conclude that it is possible to substitute synthetic antioxidants by natural ones like olive leaf phenolic compounds but we have to apply some forms of encapsulation techniques in orde

This study was conducted to determine the most effective cell disruption method and oil extraction solvent on the microalgae Neochloris oleoabundans. The three methods, either individually or combinatorial, were as integrated enzymatic hydrolysis (E) by cellulase (1, 2, 3 and 4%, v/w) and Flavourzyme (0.5, 1, 1.5 and 2% v/w); ultrasonication (U) (20 KHz and 100% spin with 20 and 40 amplitudes for 3, 8 and 13 min); and homogenization (H) (7200, 11200 and 15600 rpm for 3 min). For oil extraction, the three solvents tested were water, ethanol and methanol. The most effective method for cell disruption (as judged by quantity of subsequently extracted oil) was the integrated E+H method, followed by the integrated E+U, respectively. The fatty acids C16:0, C18:1 and C18:2 were the main constituents of extracted oil. Methanol was the most effective solvent, extracting 6.44%, 19.66% and 20.54% of fatty acids. Furthermore, in terms of biodiesel characterization, the iodic index and cetane number of the extracted oil were as 63.15 and 73.84 in ethanol, 95.33 and 62.59 in methanol and 67.66 and 76.31 in water. Therefore, the most effective method was to use the E+H method for cell disruption followed by methanol as the suitable solvent for oil extraction.