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A bacterium-like particle vaccine displaying Zika virus prM-E induces systemic immune responses in mice
  • +18
  • Hongli Jin,
  • Yujie Bai,
  • Jianzhong Wang,
  • Cui Jiao,
  • Di Liu,
  • Mengyao Zhang,
  • Entao Li,
  • Pei Huang,
  • Zhiyuan Gong,
  • Yumeng Song,
  • Shengnan Xu,
  • Na Feng,
  • Yongkun Zhao,
  • Tiecheng Wang,
  • Na Li,
  • Yuwei Gao,
  • Songtao Yang,
  • Haili Zhang,
  • Yuanyuan Li,
  • Xianzhu Xia,
  • Hualei Wang
Hongli Jin
Jilin University

Corresponding Author:[email protected]

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Yujie Bai
Jilin University
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Jianzhong Wang
Jilin Agricultural University Library
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Cui Jiao
Jilin University
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Di Liu
Jilin University
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Mengyao Zhang
Jilin University
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Entao Li
Chinese Academy of Agricultural Sciences
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Pei Huang
Jilin University
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Zhiyuan Gong
Jilin University
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Yumeng Song
Jilin University
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Shengnan Xu
Chinese Academy of Agricultural Sciences
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Na Feng
Chinese Academy of Agricultural Sciences
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Yongkun Zhao
Chinese Academy of Agricultural Sciences
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Tiecheng Wang
Chinese Academy of Agricultural Sciences
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Na Li
Chinese Academy of Agricultural Sciences
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Yuwei Gao
Chinese Academy of Agricultural Sciences
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Songtao Yang
Chinese Academy of Agricultural Sciences
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Haili Zhang
Jilin University
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Yuanyuan Li
Jilin University
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Xianzhu Xia
Jilin University
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Hualei Wang
Jilin University
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Abstract

The emergence of Zika virus (ZIKV) infection, which is unexpectedly associated with congenital defects, has prompted the development of safe and effective vaccines. The gram-positive enhancer matrix-protein anchor (GEM-PA) display system has emerged as a versatile and highly effective platform for delivering target proteins for vaccines. In this article, we developed a bacterium-like particle vaccine ZI-△-PA-GEM based on the GEM-PA system. The fusion protein ZI-△-PA, which contains the prM-E-△ protein of ZIKV (with a stem-transmembrane region deletion) and the protein anchor PA3, was expressed. The fusion protein was successfully displayed on the GEM surface, forming ZI-△-PA-GEM. Moreover, when BALB/c mice were immunized intramuscularly with ZI-△-PA-GEM combined with 201 VG and poly(I:C) adjuvants, durable ZIKV-specific IgG and protective neutralizing antibody responses were induced. Potent B cell/DC activation was also be stimulated early after immunization. Remarkably, splenocyte proliferation, the secretion of multiple cytokines, T/B cell activation and central memory T cell responses were elicited. These data indicate that ZI-△-PA-GEM is a promising bacterium-like particle vaccine candidate for ZIKV.
02 Jan 2022Submitted to Transboundary and Emerging Diseases
02 Jan 2022Submission Checks Completed
02 Jan 2022Assigned to Editor
05 Jan 2022Reviewer(s) Assigned
23 Feb 2022Review(s) Completed, Editorial Evaluation Pending
23 Feb 2022Editorial Decision: Revise Major
21 Apr 20221st Revision Received
22 Apr 2022Submission Checks Completed
22 Apr 2022Assigned to Editor
23 Apr 2022Reviewer(s) Assigned
06 May 2022Review(s) Completed, Editorial Evaluation Pending
07 May 2022Editorial Decision: Accept
Sep 2022Published in Transboundary and Emerging Diseases volume 69 issue 5. 10.1111/tbed.14594