We report a novel Cultured Neuronal Network on a chip as a viable alternative to current culture methods for the analysis of neuronal network formation and evolution of the structural properties of the network's graph . This innovative microfluidic chip fabricated from Polydimethylsiloxane, vinyl, and glass offers worthy features such as the posibility to develop and produce custom designs effortlessly, easy handling and monitoring, culture medium renewal, and reducing the exposure to contamination. Due to these benefits, longer survival of the neuronal networks are observed in comparison to conventional methods. In addition to the fast and cost-effective manufacturing of the chip, this technology provides a significant step forward in the studies of neuronal network development and many challening applications such as drug testing of in vitro cell culture models .