De novo assembly of a chromosome-level genome of naked carp (Gymnocypris
przewalskii) reveals geographic isolation of Schizothoracine fishes in
Qinghai-tibet plateau lift
Abstract
In this study, we present a de novo genome assembly of Gymnocypris
przewalski using long‐read PacBio sequencing technology with Genome-wide
high throughput chromosome conformation capture correction. The assembly
resulted in a high sequence contiguity and accuracy with 23 chromosomes
and a genome size of 945 Mb. This work is the first chromosome‐level
genome study of Schizothoracine fishes from the Qinghai-tibet plateau
(QTP). Phylogenetic analysis showed that the species differentiation
events between G. przewalskii and Cyprinus carpio occurred at 24.06 Mya
ago, which reveals geographic isolation of Schizothoracine fishes in QTP
lift; the unique gene analysis found that most of these genes enriched
in membrane transport and immune defensive pathways such as ABC
transporters, bile secretion and cell adhesion molecules. Moreover, this
genome resource is a key to understanding evolutionary adaptation to
high altitudes, disentangling complex evolutionary histories, and will
be useful for research on species diversity and resource conservation of
Schizothoracine fishes on the QTP.