The influence of the hole surface integrity on the fatigue life of 2024-T351 aluminium drilled parts was investigated. Fatigue tests were conducted on open-hole specimens and a large campaign was carried out to characterize the hole surface integrity (roughness measurements, hardness measurements, metallographic observations and residual stress analysis). An innovative technique, the Hole Opening Comparative Technique, was set up in order to analyse the residual stress state of the parts. In this study, strain hardening of the hole subsurface seems to be the main factor influencing the fatigue behaviour, associated with a residual stress state of the part.