Novel cost-efficient protein-membrane based system for cells
co-cultivation and modeling the intercellular communication
- Artem Minin
, - Igor Blatov,
- Valeria Lebedeva,
- Maxim Tuchai,
- Varvara Pozdina
, - Ilya Zubarev
Artem Minin
![](https://d197for5662m48.cloudfront.net/assets/icons/omniauth/orcid-ca37582cd91a671ed5cec57770e0e96c01b4b79b8b5b75d8598fc1ddac18c974.svg)
M N Mikheev Institute of Metal Physics of the Ural Branch of the Russian Academy of Sciences
Corresponding Author:[email protected]
Author ProfileIgor Blatov
Moscow Institute of Physics and Technology National Research University
Author ProfileValeria Lebedeva
Moscow Institute of Physics and Technology National Research University
Author ProfileVarvara Pozdina
![](https://d197for5662m48.cloudfront.net/assets/icons/omniauth/orcid-ca37582cd91a671ed5cec57770e0e96c01b4b79b8b5b75d8598fc1ddac18c974.svg)
FSBIS Institute of Immunology and Physiology of the Ural Branch of the Russian Academy of Sciences
Author ProfileIlya Zubarev
Moskovskij gosudarstvennyj universitet imeni M V Lomonosova
Author ProfileAbstract
In vitro systems serve as compact and manipulate models to investigate
interactions between different cell types. A homogeneous population of
cells predictably and uniformly responds to external factors. In a
heterogeneous cell population, the effect of external growth factors is
perceived in the context of intercellular interactions. Indirect cell
co-cultivation allows one to observe the paracrine effects of cells and
separately analyze cell populations. The article describes an
application of custom-made cell co-cultivation systems based on protein
membranes separated from the bottom of the vessel by the 3d printed
holder or kept afloat by a magnetic field. Using the proposed
co-cultivation system, we analyzed the interaction of A549 cells and
fibroblasts, in the presence and absence of growth factors. During
co-cultivation of cells, the expression of genes of the activation for
epithelial and mesenchymal transitions decreases. The article proposes
the application of a newly available system for the co-cultivation of
different cell types.13 Oct 2021Submitted to Biotechnology and Bioengineering 15 Oct 2021Submission Checks Completed
15 Oct 2021Assigned to Editor
24 Oct 2021Reviewer(s) Assigned
22 Nov 2021Editorial Decision: Revise Minor
22 Nov 2021Review(s) Completed, Editorial Evaluation Pending
19 Dec 20211st Revision Received
23 Dec 2021Submission Checks Completed
23 Dec 2021Assigned to Editor
24 Dec 2021Reviewer(s) Assigned
26 Dec 2021Editorial Decision: Accept
26 Dec 2021Review(s) Completed, Editorial Evaluation Pending