Zheng Shuo Jin

and 6 more

Background: Identification of gene polymorphisms and SNP analysis of individual patients have become crucial to select the best drug response and ensure optimal care, especially for genes directly related to asthma, such as CYSLTR1 and GSDML gene polymorphisms, an efficient and accurate detection method is helpful for the development of precision therapy. The novel detection kit we used has a reading accuracy of 99.999% for each base and the cost of detection is only 80 minutes. Objectives: In this study, a novel kit produced by Wuhan healthcare Biotechnology Co., LTD was used to detect CYSLTR1 and GSDML gene polymorphisms in asthma patients and the reliability of the novel kit was verified. Methods: The efficiency of the new kit was validated compared to gold standard, gene sequencing methods, and the close association of CYSLTR1 and GSDML SNPs with allergic asthma was also tested. As a result, the most effective drugs were obtained for patients with different gene locus expressions. Results: Identifying 367 publication participants for analysis by two different methods, we validated the reliability of the reagents with “gold standard” assays in full agreement with the DNA direct sequencing method. Our study focused on CYSLTR1 and GSDML gene polymorphisms to select the most effective drugs for asthma treatment, with leukotriene modulators being more effective in patients with the AA genotype of CYSLTR1 rs320995 and β2 agonists being more effective in patients with the TC genotype of CYSLTR1 rs320995.

Ying-Yang Xu

and 12 more

Background Allergen-specific immunotherapy (AIT) is the only disease-modifying treatment for IgE mediated disease. In china, AIT has been applied over 60 years and subcutaneous immunotherapy (SCIT) is the major route. This study analyzed the relatively comprehensive status of SCIT in our country. Methods Patients who initiated SCIT between January 1, 2015, and December 31, 2016 at Peking Union Medical College Hospital were collected and their medical records from January 1, 2015, to December 31, 2019 were obtained. The formulation and adherence of SCIT in different age groups and regions were analyzed. Results A total of 4731 patients receiving SCIT were analyzed, including 459 children (5-12 years), 624 adolescent (13-18 years) and 3648 adults. On a per-species allergen extract basis, multi-allergen SCIT were prescribed in 83% of patients. Mugwort, house dust mite and firebush were most frequently administered. On per-group allergen extract basis, 49% of SCIT were mixing formulations and weed pollen, tree pollen and house dust mite were most frequently used. The most common component of SCIT was mold in children (53%), whereas weed pollen in adolescents (59%) and adults (68%). Overall, 35% of patients completed at least 3-year SCIT. Children had the highest adherence of SCIT, followed by adolescents and adults (49%, 47% and 31%, respectively). Conclusions The majority of patients were prescribed SCIT of mixing formulation. Weed pollen was the most frequently used allergen group in SCIT, especially in Northern China. Real-life adherence in SCIT was still low which desiderate improvement of current procedure of SCIT.