Thiol/disulfide homeostasis in pericardial fluid and plasma of patients
undergoing coronary artery bypass surgery
Abstract
Background: On-pump coronary artery bypass grafting (CABG)
method affect almost all biochemical reactions by disrupting the
patient’s redox homeostasis. Detection of systemic redox hemostasis in
the patient are critical for the CABG method’s success and the prognosis
of the disease. In this study, thiol/disulfide parameters, which are
indicators of redox homeostasis, and ischemia-modified albumin (IMA)
levels in the plasma and pericardial fluid of patients who underwent
coronary artery bypass surgery were investigated. Methods:
Sixty patients who underwent an on-pump CABG operation with the
Cardiopulmonary Bypass (CPB) method were included in this study. Blood
samples were taken from the patients before and after the CPB.
Pericardial fluid samples were taken before the CPB. Then,
thiol/disulfide homeostasis, albümin, and IMA levels in the pericardial
fluid, and the patients’ plasma levels were compared. Results:
Albumin and IMA levels were significantly higher in postop compared to
preop (p < 0.001). Thiol/disulfide parameters, native thiol,
total thiol, and disulfide levels were higher and statistically
significant in preop than in postoperative examinations (p <
0.001). A negative correlation was found between pericardial fluid IMA
and thiol-disulfide parameters (p < 0.001).
Conclusions: Changes in thiol/disulfide homeostasis, albümin,
and IMA levels at different times during the on-pump CABG may be caused
by foreign non-endothelial surfaces, filters, the reperfusion process,
and pharmacological effects in the extracorporeal circulation.
Thiol/disulfide homeostasis, albumin, and IMA levels should be monitored
during the on-pump CABG and should intervene with appropriate
therapeutic strategies. In this way, secondary pathologies can be
avoided by preventing cellular damage and excessive inflammatory
responses.